Thermogenic Characterization and Antifungal Susceptibility of Candida auris by Microcalorimetry

Journal of Fungi

Mariagrazia Di Luca¹  Anna Koliszak¹  Andrej Trampuz¹  Svetlana Karbysheva¹  Jacques F. Meis²  Anuradha Chowdhary³ 

[1] BIH Center for Regenerative Therapies,Charité—Universitätsmedizin Berlin, 10117 Berlin, Germany;Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands;Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 11007, India;

关键词: candida auris;    biofilm;    antifungal resistance;    isothermal microcalorimetry;    metschnikowiaceae clade;   

DOI  :  10.3390/jof5040103

来源: DOAJ

【 摘 要 】

Candida auris has emerged globally as a multidrug-resistant fungal pathogen. Isolates of C. auris are reported to be misidentified as Candida haemulonii. The aim of the study was to compare the heat production profiles of C. auris strains and other Candida spp. and evaluate their antifungal susceptibility using isothermal microcalorimetry. The minimum heat inhibitory concentrations (MHIC) and the minimum biofilm fungicidal concentration (MBFC) were defined as the lowest antimicrobial concentration leading to the lack of heat flow production after 24 h for planktonic cells and 48 h for biofilm-embedded cells. C. auris exhibited a peculiar heat production profile. Thermogenic parameters of C. auris suggested a slower growth rate compared to Candida lusitaniae and a different distinct heat profile compared to that of C. haemulonii species complex strains, although they all belong to the Metschnikowiaceae clade. Amphotericin B MHIC and MBFC were 0.5 µg/mL and ≥8 µg/mL, respectively. C. auris strains were non-susceptible to fluconazole at tested concentrations (MHIC > 128 µg/mL, MBFC > 256 µg/mL). The heat curve represents a fingerprint of C. auris, which distinguished it from other species. Treatment based on amphotericin B represents a potential therapeutic option for C. auris infection.

【 授权许可】

Unknown