Genetic diversity, antimicrobial resistance and extended-spectrum β-lactamase type of Escherichia coli isolates from chicken, dog, pig and yak in Gansu and Qinghai Provinces, China

Journal of Global Antimicrobial Resistance

Lina Ma¹  Lu Zhao²  Kazimierz Tarasiuk³  Susan Szathmary³  Jianhua Zhou⁴  Youquan Li⁴  Xiaoan Cao⁴  Xi Lan⁴  Xuerui Li⁴  Xiaowei Gong⁴  Qiwei Chen⁴  Wenxian Jing⁴  Wen Hu⁴  Laszlo Stipkvits⁴  Yining Wang⁴  Yongsheng Liu⁵  Zygmunt Pejsak⁵ 

[1] Corresponding authors. Present address: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou 730046, Gansu, PR China.;Gansu Police Vocational College, Lanzhou 730046, Gansu, PR China;RT-Europe Research Center, Budapest, Hungary;State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, PR China;University Center of Veterinary Medicine JU-AU, PL 30-059 Kracow, AI. Mickiewicza 24/28, Poland;

关键词: Escherichia coli;    Multilocus sequence typing;    PCR phylogenetic typing;    Drug resistance;    Extended-spectrum β-lactamase;    ESBL;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: In this study, the genetic diversity, phylogenetic grouping, antimicrobial resistance and extended-spectrum β-lactamase (ESBL) types of Escherichia coli isolates from chickens, dogs, pigs and yaks in six prefectures of Gansu and Qinghai Provinces, China, were investigated. Methods: E. coli was isolated from diarrhoeic and healthy faecal samples. Multilocus sequence typing (MLST), phylogenetic grouping, antimicrobial resistance and ESBL profiles were investigated. Results: A total of 142 MLST sequence types (STs) were identified from 400 E. coli isolates. eBURST clustering analysis resolved the 142 STs into 19 clonal complexes (CCs) and 67 singletons. PCR phylogenetic typing determined the isolation rate of potentially pathogenic B2/D group isolates among all E. coli to be 12.5% from healthy animal samples and 17.5% from diarrhoeic samples. Antimicrobial susceptibility testing revealed 78 antimicrobial resistance patterns. E. coli resistance rates were highest to doxycycline, ampicillin and tetracycline, whereas polymyxin B and meropenem had the lowest resistance rates. All polymyxin B-resistant E. coli isolates were positive for the mcr-1 gene. A total of 62 ESBL-producing isolates were identified. The ESBL prevalence was 55.0% in diarrhoeic samplings and 5.6% in healthy animals. TEM (82.3%) was the predominant ESBL type, followed by CTM (43.5%) and SHV (19.4%). Conclusion: E. coli isolates in the study area have a high diversity of genetic and antimicrobial resistance patterns but a relatively low isolation rate of potentially pathogenic phylogroups. However, the somewhat high isolation rate of multidrug-resistant E. coli, particularly ESBL-producing isolates, requires continual surveillance of E. coli from animals in these areas.

【 授权许可】

Unknown