Al-Tolerant Barley Mutant hvatr.g Shows the ATR-Regulated DNA Damage Response to Maleic Acid Hydrazide

International Journal of Molecular Sciences

PaulB. Larsen¹  Jolanta Kwasniewska²  Joanna Jaskowiak²  Magdalena Rojek-Jelonek²  Iwona Szarejko³  Miriam Szurman-Zubrzycka³ 

[1] Department of Biochemistry, University of California, Riverside, CA 92521, USA;Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland;Plant Genetics and Functional Genomics Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland;

关键词: aluminum toxicity;    ATR;    barley;    cell cycle;    DDR pathway;    DNA damage;   

DOI  :  10.3390/ijms21228500

来源: DOAJ

【 摘 要 】

ATR, a DNA damage signaling kinase, is required for cell cycle checkpoint regulation and detecting DNA damage caused by genotoxic factors including Al³⁺ ions. We analyzed the function of the HvATR gene in response to chemical clastogen-maleic acid hydrazide (MH). For this purpose, the Al-tolerant barley TILLING mutant hvatr.g was used. We described the effects of MH on the nuclear genome of hvatr.g mutant and its WT parent cv. “Sebastian”, showing that the genotoxic effect measured by TUNEL test and frequency of cells with micronuclei was much stronger in hvatr.g than in WT. MH caused a significant decrease in the mitotic activity of root cells in both genotypes, however this effect was significantly stronger in “Sebastian”. The impact of MH on the roots cell cycle, analyzed using flow cytometry, showed no differences between the mutant and WT.

【 授权许可】

Unknown