DEN Open | |
Utility of autologous fecal microbiota transplantation and elucidation of microbiota in diversion colitis | |
Yosuke Tajima1  Yoshifumi Shimada1  Masato Nakano1  Hitoshi Kameyama1  Toshifumi Wakai1  Ken‐ichi Mizuno2  Kazunao Hayashi2  Takeshi Mizusawa2  Naruhiro Kimura2  Shuji Terai2  Satoshi Ikarashi2  Kentaro Tominaga2  Tomoaki Yoshida2  Nao Nakajima2  Toru Setsu2  Hiroyuki Abe2  Junji Yokoyama2  Atsunori Tsuchiya2  Hiroki Sato2  Yuichi Kojima2  Kohei Ogawa2  Yuzo Kawata2  Kazuya Takahashi2  Kentaro Oka3  Asami Matsumoto3  Motomichi Takahashi3  Ayaka Minemura3  | |
[1] Division of Digestive and General Surgery Graduate School of Medical and Dental Sciences Niigata University Niigata Japan;Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University Niigata Japan;R&D Division Miyarisan Pharmaceutical Co. Ltd. Saitama Japan; | |
关键词: colostomy; diversion colitis; Enterobacteriaceae; fecal microbiota transplantation; intestinal microbiota; | |
DOI : 10.1002/deo2.63 | |
来源: DOAJ |
【 摘 要 】
Abstract Objectives Diversion colitis (DC) is an inflammatory disorder caused by interruption of the fecal stream and subsequent nutrient deficiency from luminal bacteria. The utility of fecal microbiota transplantation (FMT) for DC was recently investigated; however, the precise pathogenesis of this condition remains unclear. This study aimed to evaluate the utility of autologous FMT in DC and to determine the related changes in the intestinal microbiota. Methods Autologous FMT was performed to reestablish the intestinal microbiota in five patients (average age, 64.6 ± 8.3 years) with DC. They underwent double‐ended colostomy. We assessed the diverted colon by endoscopy and evaluated the microbiota before and after FMT using the 16S rRNA gene sequencing method. Results All five patients had mild inflammation (ulcerative colitis endoscopic index of severity [UCEIS] 2–3) in the diverted colon based on the colonoscopic findings. Three patients presented with symptoms, such as tenesmus, mucoid stool, and bloody stool. With FMT treatment, all patients achieved endoscopic remission (UCEIS score of 0 or 1) and symptomatic improvement. We observed a significantly decreased α‐diversity in DC patients compared to healthy controls. The frequency of aerobic bacteria, such as Enterobacteriaceae, in the diverted colon decreased after autologous FMT. Conclusions This study was the first to show that the microbiota in the diverted colon was significantly affected by autologous FMT. Since interruption of the fecal stream is central to the development of DC, FMT can be considered a promising treatment.
【 授权许可】
Unknown