Diversity of Bacillus spp. from soybean phyllosphere as potential antagonist agents for Xanthomonas axonopodis pv. glycines causal of pustule disease

Biodiversitas

Rachmi Masnilah¹  Wiwiek Sri Wahyuni¹  Suhartiningsih Dwi Nurcahyanti¹  Anggi Anwar Hendra Nurdika² 

[1] Department of Plant Protection, Faculty of Agriculture, Universitas Jember. Jl. Kalimantan 37, Sumbersari, Jember 68121, East Java, Indonesia;Graduate Program of Phytopathology, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia ;

关键词: biological control;    phyllosphere bacteria;    microbial diversity;    soybean;    xanthomonas axonopodis pv. glycines;   

DOI  :  

来源: DOAJ

【 摘 要 】

Abstract. Nurcahyanti SD, Wahyuni WS, Masnilah R, Nurdika AAH. 2021. Diversity of Bacillus spp. from soybean phyllosphere as potential antagonist agents for Xanthomonas axonopodis pv. glycines causal of pustule disease. Biodiversitas 22: 5003-5011. Pustule disease caused by Xanthomonas axonopodis pv. glycines (Xag) is an important disease in soybean. Bacteria from soybean phyllosphere is one of the potential biological agents against this disease. This is because the microorganisms have similarity. This study aimed to determine the diversity and species of bacteria from the soybean phyllosphere that have potential as biological agents. The research was carried out by morphological observation, physiological - biochemical testing,  and molecularly with BOX AIR primer. Molecular identification was carried out by amplifying the 16S-rRNA gene with 27F and 1492R primers. The results showed that 11 isolates could inhibit Xag in vitro which showed morphological, biochemical, and molecular diversity. These bacteria were identified as Bacillus spp. which consisted of 4 groups, namely Bacillus siamensis, B. subtilis. B. amyloliquifaction and B. velezensis. The diversity of phyllosphere bacteria allows them to be used as biological agents because they do not inhibit each other and have diverse living abilities in various environmental conditions.

【 授权许可】

Unknown