Circulating tumor cell and cell-free RNA capture and expression analysis identify platelet-associated genes in metastatic lung cancer

BMC Cancer

Charu Aggarwal¹  Massimo Cristofanilli²  Jianming Pei³  Ranee Mehra⁴  Chandra Rao⁵  Ramillya Vlasenkova⁶  Yanis A. Boumber⁷  Ilya Serebriiskii⁷  Hossein Borghaei⁷  Tim N. Beck⁷  Patricia Fittipaldi⁸  R. Katherine Alpaugh⁸  Catherine Thrash-Bingham⁸ 

[1] Abramson Cancer Center and Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Perelman School of Medicine;Feinberg School of Medicine, Robert H Lurie Comprehensive Cancer Center;Genomics Facility, Fox Chase Cancer Center;Head and Neck Medical Oncology, University of Maryland Greenebaum Comprehensive Cancer Center;Janssen Diagnostics LLC;Kazan Federal University;Program in Molecular Therapeutics, Fox Chase Cancer Center;Protocol Support Laboratory, Fox Chase Cancer Center;

关键词: NSCLC;    SCLC;    Circulating tumor cells;    Cell-free RNA;    Platelets;   

DOI  :  10.1186/s12885-019-5795-x

来源: DOAJ

【 摘 要 】

Abstract Background Circulating tumor cells (CTC) and plasma cell-free RNA (cfRNA) can serve as biomarkers for prognosis and treatment response in lung cancer. One barrier to the selected or routine use of CTCs and plasma cfRNA in precision oncology is the limited quantity of both, and CTCs are only seen in metastatic disease. As capture of CTCs and plasma cfRNA presents an opportunity to monitor and assess malignancies without invasive procedures, we compared two methods for CTC capture and identification, and profiled mRNA from CTCs and plasma cfRNA to identify potential tumor-associated biomarkers. Methods Peripheral blood was collected from ten patients with small cell lung cancer (SCLC), ten patients with non-small cell lung cancer (NSCLC) and four healthy volunteers. Two methods were used for CTC capture: the standard epithelial cell adhesion molecule (EpCam) CellSearch kit (unicapture) and EpCAM plus HER2, EGFR and MUC-1 specific combined ferrofluid capture (quadcapture). For the quadcapture, anti-cytokeratin 7 (CK7) was additionally used to assist in CTC identification. NanoString analysis was performed on plasma cfRNA and on mRNA from combined ferrofluid isolated CTCs. Expression data was analyzed using STRING and Reactome. Results Unicapture detected CTCs in 40% of NSCLC and 60% of SCLC; whereas, quadcapture/CK7 identified CTCs in 20% of NSCLC and 80% of SCLC. Bioinformatic analysis of NanoString data identified high expression of a platelet factor 4 (PF4)-related group of transcripts. Conclusions Quadcapture ferrofluid reagent did not significantly improve CTC capture efficacy. NanoString analysis based on CTC and plasma cfRNA data highlighted an intriguing PF-4-centric network in patients with metastatic lung cancer.

【 授权许可】

Unknown