期刊论文详细信息
Antibiotics
Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy
Antonella Pocognoli1  Lucia Brescini2  Andrea Brenciani2  Simona Fioriti2  Andrea Giacometti2  Federica Salari2  Oscar Cirioni2  Gianluca Morroni2  Marina Mingoia2  Eleonora Giovanetti3  Annamaria Offidani4  Elisa Molinelli4  Oriana Simonetti4 
[1] Clinical Microbiology Laboratory, “Ospedali Riuniti”, 60126 Ancona, Italy;Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy;Department of Life and Environmental Sciences, Polytechnic University of Marche, 60126 Ancona, Italy;Dermatological Clinic, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy;
关键词: ceftaroline;    MRSA;    penicillin-binding proteins;    SCCmec;   
DOI  :  10.3390/antibiotics10081026
来源: DOAJ
【 摘 要 】

Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCCmecI, ST22/CC22-t18014-SCCmecIV, and ST22/CC22-t022-SCCmecIV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCCmecI strains and all the other PBPs for ST22-t18014-SCCmecIV and ST22-t022-SCCmecIV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.

【 授权许可】

Unknown   

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