Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

Antibiotics

Antonella Pocognoli¹  Lucia Brescini²  Andrea Brenciani²  Simona Fioriti²  Andrea Giacometti²  Federica Salari²  Oscar Cirioni²  Gianluca Morroni²  Marina Mingoia²  Eleonora Giovanetti³  Annamaria Offidani⁴  Elisa Molinelli⁴  Oriana Simonetti⁴ 

[1] Clinical Microbiology Laboratory, “Ospedali Riuniti”, 60126 Ancona, Italy;Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, Italy;Department of Life and Environmental Sciences, Polytechnic University of Marche, 60126 Ancona, Italy;Dermatological Clinic, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy;

关键词: ceftaroline;    MRSA;    penicillin-binding proteins;    SCCmec;   

DOI  :  10.3390/antibiotics10081026

来源: DOAJ

【 摘 要 】

Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCCmecI, ST22/CC22-t18014-SCCmecIV, and ST22/CC22-t022-SCCmecIV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCCmecI strains and all the other PBPs for ST22-t18014-SCCmecIV and ST22-t022-SCCmecIV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.

【 授权许可】

Unknown