Phytochemistry and toxicological assessment of Bryonia dioica roots used in north-African alternative medicine

Open Chemistry

Bari Ahmed¹  Ali Syed Saeed¹  khlil Naima²  Bourhia Mohammed²  Benbacer Laila³ 

[1] Central Laboratory, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, RiyadhSaudi Arabia;Laboratory of chemistry, Biochemistry nutrition, and environment, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca, Morocco;Life Science Division. National Centre for Energy, Sciences, and Nuclear Techniques. B.P 1382 RP, 10001Rabat, Morocco;

关键词: acute toxicity;    sub-acute toxicity;    histopathology;    biochemistry;    gc-ms;   

DOI  :  10.1515/chem-2019-0150

来源: DOAJ

【 摘 要 】

To investigate the phytochemical composition, acute and sub-acute toxicity of the aqueous extract of B. dioica roots. The phytochemical analysis was performed using gas chromatography-mass spectrometry (GC-MS). The acute toxicity of the aqueous extract of B.dioica roots was assessed in mice with single doses ranging from 250 to 1000 mg/kg for 14 days. The sub-acute toxicity was carried out with repeated doses ranging from 64.5 to 250 mg/kg for 28 days. Histopathological changes and markers of renal and liver function were investigated. The results of GC-MS analysis showed the presence of interesting phytoconstituents. The clinical symptoms and mortalities that occurred in treated mice were more remarkable due to the increasing sample concentration of the studied extract. However, no mortalities, or histopathological, or biochemical disturbances were observed even at the maximal dose administered (250 mg/kg). The outcome of the present work suggests that the treatment of animals with single doses of B. dioica roots extract higher than 250 mg/kg produces significant toxicities, however, treatment with repeated doses up to 250 mg/kg for 28 days seems to be safe for animals.

【 授权许可】

Unknown