Application of Single-Radial Immuno-diffusion test for evaluation of uropathogenic Escherichia coli sonicated immunogen in chick embryo

Mirror of Research in Veterinary Sciences and Animals

Zina bakir¹  Walaa Frhan O¹  Sabaa Helal H²  Abdulazeez Akram H³ 

[1] Al-Qassim Green University /Babylon –Iraq;Department of Internal and Preventive Medicine, College of Veterinary Medicine, AL. Qasim Green University, Ira;Department of Research, Ministry of science and technology, Iraq;

关键词: Uropathogenic E. coli;    WSC Antigen;    sonicated immunogen.;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study intended to evaluate the effect of whole cell sonicated uropathogenic Escherichia coli antigen (WCS.Ag) against the challenged of the infectious dose 50 (ID50) in chicken embryos. Twenty chicken embryos / one day age were divided into 2 equal groups. The first group was inoculated with 0.1 ml of WSC, Ag, the second group (control) was injected with 0.1 ml phosphate buffer saline (PBS). Blood samples were collected at 2 and 4weeks after inoculations. Single Radial Immuno-Diffusion assay (SRID) was used to test the significance of the effects of treatments. Fifty groups of eggs (10 eggs per group) were used to determine the infectious dose 50 (ID50). All chicken embryos of the treated and control groups were challenged with 3ID50 (2×106) of virulent uropathogenic Escherichia coli. The SRIT showed significant increase in antibody titers in the experimental group compared with the control group. The post challenge with ID50 of E. coli revealed that all chicken embryos in control group suffered from severe clinical signs of colibacillosis. In conclusion, this study indicated the ability of WSC. Ag to protect chick embryo against the challenged of the infectious dose 50 (ID50).

【 授权许可】

Unknown