Salinity Stress Enhances the Antioxidant Capacity of Bacillus and Planococcus Species Isolated From Saline Lake Environment

Frontiers in Microbiology

Dalal Hussien M. Alkhalifah²  Wael N. Hozzein³  Ahmed S. M. Mousa³  Hamada AbdElgawad³  Sulaiman A. Al Yousef⁴  Abdelrahim H. A. Hassan⁵  Gerrit T. S. Beemster⁶ 

[1] Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia;Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt;Department of Clinical Laboratory Science, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin, Saudi Arabia;Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt;Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerp, Belgium;

关键词: salinity stress;    Bacillus;    Planococcus;    stress markers;    antioxidants;   

DOI  :  10.3389/fmicb.2020.561816

来源: DOAJ

【 摘 要 】

This study aims at exploiting salinity stress as an innovative, simple, and cheap method to enhance the production of antioxidant metabolites and enzymes from bacteria for potential application as functional additives to foods and pharmaceuticals. We investigated the physiological and biochemical responses of four bacterial isolates, which exhibited high tolerance to 20% NaCl (wt/vol), out of 27 bacterial strains isolated from Aushazia Lake, Qassim region, Saudi Arabia. The phylogenetic analysis of the 16S rRNA genes of these four isolates indicated that strains ST1 and ST2 belong to genus Bacillus, whereas strains ST3 and ST4 belong to genus Planococcus. Salinity stress differentially induced oxidative damage, where strains ST3 and ST4 showed increased lipid peroxidation, lipoxygenase, and xanthine oxidase levels. Consequently, high antioxidant contents were produced to control oxidative stress, particularly in ST3 and ST4. These two Planococcus strains showed increased glutathione cycle, phenols, flavonoids, antioxidant capacity, catalase, and/or superoxide dismutase (SOD). Interestingly, the production of glutathione by Planococcus strains was some thousand folds greater than by higher plants. On the other hand, the induction of antioxidants in ST1 and ST2 was restricted to phenols, flavonoids, peroxidase, glutaredoxin, and/or SOD. The hierarchical analysis also supported strain-specific responses. This is the first report that exploited salinity stress for promoting the production of antioxidants from bacterial isolates, which can be utilized as postbiotics for promising applications in foods and pharmaceuticals.

【 授权许可】

Unknown