Amino Acid Deletions in p6Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding

International Journal of Molecular Sciences

Yen-Hsu Chen¹  Sheng-Fan Wang¹  Chih-Yen Lin¹  Po-Liang Lu¹  AspiroNayim Urbina¹  Wen-Hung Wang¹  Chun-Sheng Yeh²  Ruei-Yu Yuan²  Yi-MingArthur Chen³  Fu-Tong Liu⁴ 

[1] Center for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;Institute of Biomedical Sciences, Academia Sinica 11529, Taiwan;

关键词: Galectin-3;    HIV-1;    CRF07_BC;    p6^Gag;    Alix;   

DOI  :  10.3390/ijms21082910

来源: DOAJ

【 摘 要 】

HIV-1 CRF07_BC is a recombinant virus with amino acid (a.a.) deletions in p6^Gag, which are overlapped with the Alix-binding domain. Galectin-3 (Gal3), a β-galactose binding lectin, has been reported to interact with Alix and regulate HIV-1 subtype B budding. This study aims to evaluate the role of Gal3 in HIV-1 CRF07_BC infection and the potential effect of a.a. deletions on Gal3-mediated regulation. A total of 38 HIV-1+ injecting drug users (IDUs) were enrolled in the study. Viral characterization and correlation of Gal3 were validated. CRF07_BC containing 7 a.a. deletions and wild-type in the p6^Gag (CRF07_BC-7d and -wt) were isolated and infectious clones were generated. Viral growth kinetic and budding assays using Jurkat-CCR5/Jurkat-CCR5-Gal3 cells infected with CRF07_BC were performed. Results indicate that 69.4% (25/38) of the recruited patients were identified as CRF07_BC, and CRF07_BC-7d was predominant. Slow disease progression and significantly higher plasma Gal3 were noted in CRF07_BC patients (p < 0.01). Results revealed that CRF07_BC infection resulted in Gal3 expression, which was induced by Tat. Growth dynamic and budding assays indicated that Gal3 expression in Jurkat-CCR5 cells significantly enhanced CRF07_BC-wt replication and budding (p < 0.05), while the promoting effect was ameliorated in CRF07_BC-7d. Co-immunoprecipitation found that deletions in the p6^Gag reduced Gal-3-mediated enhancement of the Alix–Gag interaction.

【 授权许可】

Unknown