Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction

Bioengineering

Juan Pablo Lamas¹  Marta Sanchez-Nande¹  Carmen Garcia-Jares¹  Marta Lores¹ 

[1] Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain;

关键词: Camellia seeds;    fatty acids;    limonene;    matrix solid-phase dispersion;    GC-MS;   

DOI  :  10.3390/bioengineering4040087

来源: DOAJ

【 摘 要 】

Camellia (genus of flowering plants of fam. Theaceae) is one of the main crops in Asia, where tea and oil from leaves and seeds have been utilized for thousands of years. This plant is excellently adapted to the climate and soil of Galicia (northwestern Spain) and northern Portugal where it is grown not only as an ornamental plant, but to be evaluated as a source of bioactive compounds. In this work, the main fatty acids were extracted from Camellia seeds of four varieties of Camellia: sasanqua, reticulata, japonica and sinensis, by means of matrix-solid phase dispersion (MSPD), and analyzed by gas chromatography (GC) with MS detection of the corresponding methyl esters. MSPD constitutes an efficient and greener alternative to conventional extraction techniques, moreover if it is combined with the use of green solvents such as limonene. The optimization of the MSPD extraction procedure has been conducted using a multivariate approach based on strategies of experimental design, which enabled the simultaneous evaluation of the factors influencing the extraction efficiency as well as interactions between factors. The optimized method was applied to characterize the fatty acids profiles of four Camellia varieties seeds, allowing us to compare their fatty acid composition.

【 授权许可】

Unknown