One Step Assembly of Thin Films ofCarbon Nanotubes on Screen Printed Interface for Electrochemical Aptasensing of BreastCancer Biomarker

Sensors

Jean Louis Marty¹  Gaelle Catanante¹  Muhammad Azhar Hayat Nawaz²  Mian Hasnain Nawaz²  Akhtar Hayat²  Sajid Rauf²  Gilvanda Nunes³ 

[1] BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France;Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan;Technological Chemistry Department, Federal University of Maranhão, CCET/UFMA, Av. Portugueses, Cidade Universitária do Canga, São Luis 65080-040, MA, Brazil;

关键词: thin films;    carbon nanotubes;    high density;    electrochemical aptasensor;    cancer diagnosis;   

DOI  :  10.3390/s16101651

来源: DOAJ

【 摘 要 】

Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a verywell-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer–Emmett– Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed methodology makes use of a minimum amount of CNTs and toxic solvents, and is successfully demonstrated to form thin films over macroscopic areas of screen printed carbon transducer surface. The CNT coated screen printed transducer surface was integrated in the fabrication of electrochemical aptasensors for breast cancer biomarker analysis. This CNT coated platform can be applied to immobilize enzymes, antibodies and DNA in the construction of biosensor for a broad spectrum of applications.

【 授权许可】

Unknown