Effect of the Most Relevant CYP3A4 and CYP3A5 Polymorphisms on the Pharmacokinetic Parameters of 10 CYP3A Substrates

Biomedicines

Cristina Rodríguez-Antona¹  María Santos¹  AlbertoM. Borobia²  Dolores Ochoa³  Susana Almenara³  Dora Koller³  Marcos Navares-Gómez³  Pablo Zubiaur³  Miriam Saiz-Rodríguez³  Manuel Román³  Francisco Abad-Santos³  Gina Mejía³ 

[1] Centro Nacional de Investigaciones Oncológicas (CNIO), 28029 Madrid, Spain;Clinical Pharmacology Department, Hospital Universitario La Paz, 28029 Madrid, Spain;Clinical Pharmacology Department, Hospital Universitario de La Princesa, Instituto Teófilo Hernando, Universidad Autónoma de Madrid (UAM), Instituto de Investigación Sanitaria La Princesa (IP), 28006 Madrid, Spain;

关键词: CYP3A4;    CYP3A5;    pharmacokinetics;   

DOI  :  10.3390/biomedicines8040094

来源: DOAJ

【 摘 要 】

Several cytochrome P450 (CYP) CYP3A polymorphisms were associated with reduced enzyme function. We aimed to evaluate the influence of these alleles on the pharmacokinetic parameters (PK) of several CYP3A substrates. We included 251 healthy volunteers who received a single dose of ambrisentan, atorvastatin, imatinib, aripiprazole, fentanyl, amlodipine, donepezil, olanzapine, fesoterodine, or quetiapine. The volunteers were genotyped for CYP3A4 and CYP3A5 polymorphisms by qPCR. To compare the PK across studies, measurements were corrected by the mean of each parameter for every drug and were logarithmically transformed. Neither CYP3A phenotype nor individual CYP3A4 or CYP3A5 polymorphisms were significantly associated with differences in PK. However, regarding the substrates that are exclusively metabolized by CYP3A, we observed a higher normalized AUC (p = 0.099) and a tendency of lower normalized Cl (p = 0.069) in CYP3A4 mutated allele carriers what was associated with diminished drug metabolism capacity. CYP3A4 polymorphisms did not show a pronounced influence on PK of the analysed drugs. If so, their impact could be detectable in a very small percentage of subjects. Although there are few subjects carrying CYP3A4 double mutations, the effect in those might be relevant, especially due to the majority of subjects lacking the CYP3A5 enzyme. In heterozygous subjects, the consequence might be less noticeable due to the high inducible potential of the CYP3A4 enzyme.

【 授权许可】

Unknown