Rapid Fabrication of Cell-Laden Microfibers for Construction of Aligned Biomimetic Tissue

Frontiers in Bioengineering and Biotechnology

Mingfeng Li³  Ting Zhang³  Bingchuan Lu³  Yongcong Fang³  Zibo Liu³  Zhuo Xiong³ 

[1] Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing, China;Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing, China;“Biomanufacturing and Engineering Living Systems” Innovation International Talents Base (111 Base), Beijing, China;

关键词: wet spinning;    cell-laden microfiber;    core-sheath microfiber;    hollow microfiber;    microfiber assembling;   

DOI  :  10.3389/fbioe.2020.610249

来源: DOAJ

【 摘 要 】

Bottom-up engineering of tissue constructs is being rapidly developed and broadly applied in biomanufacturing. As one type of building block, cell-laden microfibers are promising for reconstruction of oriented structures and functions of linear tissues, such as skeletal muscles, myocardia, and spinal cord tissues. Herein, we propose wet-spinning method with agitating collection, wherein alginate-based material is extruded into an agitated CaCl2 bath with a magnetic rotor acting as the microfiber collector. By applying this method, we achieve rapid fabrication and oriented collection of hydrogel microfibers with diameters ranging from 100 to 400 μm. In addition, we encapsulate myoblasts in the hydrogel to form cell-laden microfibers, which show a high viability (more than 94%) during in vitro culture. Moreover, the method allows to fabricate of cell-laden core–sheath microfibers and hollow microfibers. We also fabricate 3D constructs using various methods of microfiber assembly like weaving and braiding. The assembling results suggest that the proposed method is a promising technology for bottom-up engineering of aligned biomimetic tissue constructs.

【 授权许可】

Unknown