A Deoxyuridine-Based Far-Red Emitting Viscosity Sensor

Molecules

Xiling Yue¹  Yuanwei Zhang¹  Mengyuan Wang¹  Sheng Yao¹  Mykhailo V. Bondar²  Kevin D. Belfield³ 

[1] Department of Chemistry, University of Central Florida, P.O. Box 162366, Orlando, FL 32816, USA;Institute of Physics, National Academy of Sciences of Ukraine, Prospect Nauki, 46, Kiev-28 03028, Ukraine;School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, China;

关键词: microviscosity sensor;    far-red fluorescent probe;    bioimaging;    nucleosides;    Sonogashira coupling;    molecular rotor;   

DOI  :  10.3390/molecules21060709

来源: DOAJ

【 摘 要 】

A novel deoxyuridine (dU) benzothiazolium (BZ) derivative, referred to as dU-BZ, is reported that was synthesized via Sonogashira coupling reaction methodology. The deoxyuridine building block was introduced to enhance hydrophilicity, while an alkynylated benzothiazolium dye was incorporated for long wavelength absorption to reduce potential phototoxicity that is characteristic of using UV light to excite common fluorphores, better discriminate from native autofluorescence, and potentially facilitate deep tissue imaging. An impressive 30-fold enhancement of fluorescence intensity of dU-BZ was achieved upon increasing viscosity. Fluorescence quantum yields in 99% glycerol/1% methanol (v/v) solution as a function of temperature (293–343 K), together with viscosity-dependent fluorescence lifetimes and radiative and non-radiative rate constants in glycerol/methanol solutions (ranging from 4.8 to 950 cP) were determined. Both fluorescence quantum yields and lifetimes increased with increased viscosity, consistent with results predicted by theory. This suggests that the newly-designed compound, dU-BZ, is capable of functioning as a probe of local microviscosity, an aspect examined by in vitro bioimaging experiments.

【 授权许可】

Unknown