Infection with different strains of SARS-COV-2 in patients with COVID-19

Archives of Biological Sciences

Hassan Safa A.¹  Al-Shuhaib Mohammed Baqur S.²  Alhassnawi Alaa T.S.³  Hashim Hayder O.⁴  Mousa Mazin J.⁴  Mohammed Mudher K.⁵  Abdulameer Hadeer H.⁶ 

[1] Alfadhel Training and Development Company / Babylon Branch,Iraq;Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq;Department of Biology, College of Science, University of Babylon, Babil, Iraq;Department of Clinical Laboratory Sciences, College of Pharmacy, University of Babylon, Babil, Iraq;Department of Pharmacy, Al-Manara College of Medical Science, Iraq;University of Kufa, Faculty of Education for Girls,Iraq;

关键词: co-infection;    sars-cov-2;    covid-19;    spike glycoprotein;    stop mutations;   

DOI  :  10.2298/ABS201024051H

来源: DOAJ

【 摘 要 】

The biological diversity of SARS-CoV-2 was assessed by investigating the genetic variations of the spike glycoprotein of patients with COVID-19 in Iraq. Sequencing identified fifteen novel nucleic acid variations with a variety of distributions within the investigated samples. The electropherograms of all identified variations showed obvious co-infections with two different viral strains per sample. Most samples exhibited three nonsense single nucleotide polymorphism (SNPs), p.301Cdel, p.380Ydel and p.436del, which yielded three truncated spike glycoproteins, respectively. Network and phylogenetic analyses indicated that all viral infections were derived from multiple viral origins. Results inferred from the specific clade-based tree showed that some viral strains were derived from European G-clade sequences. Our data demonstrated the absence of single-strain infection among all investigated samples in the studied area, which entails a higher risk of SARS-CoV-2 in this country. The identified high frequency of truncated spike proteins suggests that defective SARS-CoV-2 depend on helper strains possessing intact spikes during infection. Alternatively, another putative ACE2-independent route of viral infection is suggested. To the best of our knowledge, this is the first report to describe co-infection with multiple strains of SARS-CoV-2 in patients with COVID-19.

【 授权许可】

Unknown