Hepatoprotective effects of extracts, fractions and compounds from the stem bark of Pentaclethra macrophylla Benth: Evidence from in vitro and in vivo studies

Biomedicine & Pharmacotherapy

Télesphore Benoit Nguelefack¹  Agathe Lambou Fotio²  Jonas Kühlborn³  Till Opatz³  Mathias Kenfack Tsague⁴  Elvine Pami Mbuyo-Nguelefack⁴  Cyrille Lionel Kamga Bomgning⁴  Beaudelaire Kemvoufo Ponou⁵  Borice Tapondjou Tsafack⁵  Pierre Valery Kemdoum Sinda⁵  Léon Azefack Tapondjou⁵  Rémy Bertrand Teponno⁵ 

[1] Corresponding authors.;Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon;Johannes Gutenberg University Mainz, Department of Chemistry, Duesbergweg 10-14, D–55128, Mainz, Germany;Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Research Unit of Environmental and Applied Chemistry, Faculty of Science, University of Dschang, Box 67, Dschang, Cameroon;

关键词: Pentaclethra macrophylla;    Isolated compounds;    Hepatoprotection;    Structure-activity relationships;    Lipid peroxidation;    GaIN/LPS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Aim: To identify the bioactive hepatoprotective components of the ethanol extract of Pentaclethra macrophylla stem bark using in vitro and in vivo approaches. Methods: The bioguided-fractionation of the ethanol extract was based on the substances’ capacity to prevent in vitro, the lipid peroxidation of hepatocytes’ membranes induced by hydrogen peroxide. For the in vivo hepatoprotective test, mice were treated orally with the ethyl acetate (EtOAc) fraction of the ethanol extract at doses of 50 and 75 mg/kg/day for one week and subjected to d-galactosamine/lipopolysaccharide (GaIN/LPS)-induced hepatotoxicity. Blood samples were collected for alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), TNF-α and IL-1β assays. The liver was harvested for histological and biochemical (proteins, glutathione (GSH), catalase and superoxide dismutase (SOD)) analysis. Results: The ethanol extract and fractions induced concentration-dependent inhibition of lipid peroxidation (IC50: 3.21–48.90 μg/mL) greater than that of silymarin (IC50: 117.4 μg/mL). The purification of the sub-fractions of EtOAc fraction yielded: (7R)-7-hydroxyhexacosanoic acid (1), (7R)-1-(7-hydroxyhexacosanoyl) glycerol (2), bergenin (3), 11-O-galloylbergenin (4), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol (5), β-sitosterol 3-O-β-d-glucopyranosyl (6) and β-sitosterol (7)), among which 11-O-galloylbergenin (IC50:1.8 μg/mL) was the most effective. The EtOAc fraction significantly reduced the serum level of ALAT, ASAT and TNF-α in vivo. This EtOAc fraction increased the liver protein content and protected the liver against structural damages, but did not boost the endogenous antioxidant parameters. Conclusion: The stem bark of Pentaclethra macrophylla possesses hepatoprotective effects that may result from its capacity to inhibit lipid peroxidation and could be attributed to its active components 3, 4 and 2.

【 授权许可】

Unknown