In vitro release and cytotoxicity study of encapsulated sulfasalazine within LTSP micellar/liposomal and TSP micellar/niosomal nano-formulations

Alexandria Engineering Journal

Mohammed F. Aldawsari¹  Mohamad Taleuzzaman¹  Md Lutfor Rahman²  Mohammed Asadullah Jahangir³  Asieh Yahyazadeh⁴  Mohd Sani Sarjadi⁵  Mohd Yasir⁶  Nosrat O Mahmoodi⁷  Alireza Soltani⁸  Ameeduzzafar Zafar⁹  Mohammad Javed Ansari⁹  Leila Daneshmandi^1,10  Vahid Erfani-Moghadam^1,11 

[1] Corresponding author at: Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.;Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran;Golestan Rheumatology Research Center, Golestan University of Medical Science, Gorgan, Iran;Cancer Research Center, Golestan University of Medical Sciences, Gorgan, Iran;Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA;Department of Chemistry, Faculty of Science, University of Guilan, P.O. Box 41335-1914, Rasht, Iran;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Maulana Azad University, Jodhpur, Rajasthan, India;Department of Pharmaceutics, College of Pharmacy, Jouf University, Sakaka, Aljouf, Saudi Arabia;Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia;Department of Pharmaceutics, Nibha Institute of Pharmaceutical Sciences, Rajgir 803116, Bihar, India;Department of Pharmacy, College of Health Science, Arsi University, Asella, Ethiopia;

关键词: Micelles;    Niosomes;    Liposomes;    Sulfasalazine;    Drug Delivery;    Bioavailability;   

DOI  :  

来源: DOAJ

【 摘 要 】

The micelles/liposome formulation for the first time has been constructed via thin-film hydration method containing soy lecithin (L), tween 80 (T), squalene (S), and polyvinyl alcohol (P) (LTSP nanoparticles). Similar ingredients except for lecithin were used for preparing micellar/niosomal vesicular SSZ nanoformulation (TSP nanoparticles). The percent drug loading and encapsulation efficiency of SSZ was 7.39% and 98.5 ± 0.3 % for the 7.5:100 (w/w) ratio of SSZ: total weight of LTSP, while the percent drug loading and encapsulation efficiency of SSZ was 4.7% and 62.85 ± 0.3 % in the TSP nanoformulation. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) results showed that both formulations formed spherical micelles and vesicles with globule sizes of 25 ± 1.2 nm and 100 ± 20.5 nm respectively. The cell toxicity evaluations showed that both LTSP and TSP nanoformulations without drug were nontoxic (at the range of this experiment) for Human Dermal Fibroblasts (HDF) as a normal cell line but SSZ loaded nanoformulation exhibited increased cell toxicity with half-maximal inhibitory concentration (IC50) of 940 µM for SSZ alone to near 240 µM for SSZ loaded nanoformulation (approximately four times). In vitro release experiments exhibited sustained release of SSZ from both nanoformulations. The LTSP micellar/liposomal and TSP micellar/niosomal nanoformulation for SSZ delivery can be considered as appropriate approaches for improving its bioavailability and probably they are good candidates for future clinical investigations.

【 授权许可】

Unknown