Cloning and Characterization of Polyhydroxybutyrate Synthase from Methylobacterium extorquens AM1

Журнал Сибирского федерального университета: Серия Биология

Dmitry N. Fedorov¹  Yuri A. Trotsenko¹  Nina V. Doronina¹  Svetlana A. Zamakhaeva¹ 

[1] G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS 5 Prospect Nauki, Pushchino, Moscow region, 142290, Russia;

关键词: Methylobacterium extorquens;    phaC;    methylotrophs;    polyhydroxybutyrate;    PHB-synthase;    Methylobacterium extorquens;    phaC;   

DOI  :  

来源: DOAJ

【 摘 要 】

Multiple genes encoding putative PHB synthases (up to 5 in single strain) were found in Methylobacterium genomes. As a result of phylogenetic analysis proteins PhaC1, PhaC2, PhaC3 were identified as class I PHB synthases, PhaC4 proteins were identified as class III PHB synthases, while PhaC5 apparently belongs to uncharacterized class of PHB synthases. Firstly, the recombinant class I PBH synthase (EC 2.3.1.B2) encoded by phaC1 gene from Methylobacterium extorquens AM1 was purified and characterized. Molecular mass of enzyme monomer was 78 kDa. Michaelis constant (Km) for PhaC1 was 1,3 mM and maximal reaction rate (Vmax) was 0,1 μmol∙min–1∙mg–1. The deletion mutant of Methylobacterium extorquens in the phaC gene was obtained which is promising for further study of peculiarities of methylobacteria’s PHB biosynthesis

【 授权许可】

Unknown