Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region

Turkish journal of biology

article

ZEYNEP DEMİR ÖKSÜZ¹  TUĞRUL DORUK¹  NEVİN YAĞCI³  SEDEF TUNCA GEDİK¹ 

[1] Molecular Biology and Genetic Department, Faculty of Science, Gebze Technical University;Molecular Biology and Genetic Department, Faculty of Arts and Science, Ondokuz Mayıs University;Department of Environmental Engineering, Faculty of Civil Engineering, İstanbul Technical University

关键词: Streptomyces coelicolor;    SCO7613;    polyhydroxyalkanoate synthase;    phaC;    glycerol;   

DOI  :  10.3906/biy-2011-16

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, pha C gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. BLAST search results of the GenBank database argued that SCO7613 could specify a putative polyhydroxyalkanoate synthase (PhaC) responsible for PHA biosynthesis. Deduced amino acid sequence of SCO7613 showed the presence of conserved lipase box like sequence, 555 GASAG 559 , in which serine residue was present as the active nucleophile. Present study describes deletion of putative S. coelicolor pha C gene via PCR dependent method. We showed that SCO7613 is not an essential gene in S. coelicolor and its deletion affected PHA accumulation negatively although it is not ceased. Transcomplementation abolished the mutant phenotype, demonstrating that the decrease in PHA resulted from the deletion of SCO7613.

【 授权许可】

Unknown