Antiviral Activity of Ribavirin against Tilapia tilapinevirus in Fish Cells

Pathogens

Tuchakorn Lertwanakarn¹  Puntanat Tattiyapong²  Pirada Trongwongsa²  Sangchai Yingsakmongkol²  Win Surachetpong²  Matepiya Khemthong² 

[1] Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand;Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand;

关键词: Tilapia tilapinevirus;    Tilapia lake virus;    antivirus;    ribavirin;    tilapia;    TiLV;   

DOI  :  10.3390/pathogens10121616

来源: DOAJ

【 摘 要 】

The outbreak of the novel Tilapia tilapinevirus or Tilapia lake virus (TiLV) is having a severe economic impact on global tilapia aquaculture. Effective treatments and vaccines for TiLV are lacking. In this study, we demonstrated the antiviral activity of ribavirin against TiLV in E-11 cells. Our findings revealed that at concentrations above 100 μg/mL, ribavirin efficiently attenuates the cytopathic effect of the TiLV infection in fish cells. When administered in a dose-dependent manner, ribavirin significantly improved cell survival compared to the untreated control cells. Further investigation revealed that the cells exposed to ribavirin and TiLV had a lower viral load (p < 0.05) than the untreated cells. However, at concentrations above 1000 μg/mL, ribavirin led to cell toxicity. Taken together, our results demonstrate the efficacy of this antiviral drug against TiLV and could be a useful tool for future research on the pathogenesis and replication mechanism of TiLV as well as other piscine viruses.

【 授权许可】

Unknown