Evolutionary consequences of intra-patient phage predation on microbial populations

eLife

Jessica E Teng¹  Jacques Boncy²  Andrew Camilli²  Kimberley D Seed³  Minmin Yen³  Richelle C Charles⁴  B Jesse Shapiro⁵  Isabelle J Hilaire⁶  Louise C Ivers⁶  Jason B Harris⁶ 

[1] Department of Medicine, Harvard Medical School, Boston, United States;Division of Global Health Equity, Brigham and Women's Hospital, Boston, United States;Department of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, United States;Division of Infectious Diseases, Massachusetts General Hospital, Boston, United States;Département de sciences biologiques, Université de Montréal, Montreal, Canada;Partners In Health, Boston, United States;

关键词: Vibrio cholerae;    cholera;    bacteriophage;    phage;    OmpU;    ToxR;   

DOI  :  10.7554/eLife.03497

来源: DOAJ

【 摘 要 】

The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen during the natural course of infection within individual patients. We analyzed geographically and temporally disparate cholera patient stool samples from Haiti and Bangladesh and found that phage predation can drive the genomic diversity of intra-patient V. cholerae populations. Intra-patient phage-sensitive and phage-resistant isolates were isogenic except for mutations conferring phage resistance, and moreover, phage-resistant V. cholerae populations were composed of a heterogeneous mix of many unique mutants. We also observed that phage predation can significantly alter the virulence potential of V. cholerae shed from cholera patients. We provide the first molecular evidence for predatory phage shaping microbial community structure during the natural course of infection in humans.

【 授权许可】

Unknown