A Suggested New Bacteriophage Genus, “Kp34likevirus”, within the Autographivirinae Subfamily of Podoviridae

Viruses

Harald Eriksson⁶  Barbara Maciejewska⁵  Agnieszka Latka⁵  Grazyna Majkowska-Skrobek⁵  Marios Hellstrand⁶  Öjar Melefors⁴  Jin-Town Wang¹  Andrew M. Kropinski²  Zuzanna Drulis-Kawa⁵  Anders S. Nilsson⁶  Abram Aertsen³ 

[1] Department of Microbiology, National Taiwan University, College of Medicine, Taipei 10051, Taiwan; E-Mail:;Departments of Food Science, Molecular & Cellular Biology, and Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada; E-Mail:Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden;;Department of Microbiology, Public Health Agency of Sweden, Solna 171 82, Sweden; E-Mail:;Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland; E-Mails:;Department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden; E-Mails:

关键词: “Kp34likevirus”;    phikmvlikevirus;    Autographivirinae;    Klebsiella pneumoniae;    bacteriophage;    phage;    Podoviridae;   

DOI  :  10.3390/v7041804

来源: mdpi

【 摘 要 】

Klebsiella pneumoniae phages vB_KpnP_SU503 (SU503) and vB_KpnP_SU552A (SU552A) are virulent viruses belonging to the Autographivirinae subfamily of Podoviridae that infect and kill multi-resistant K. pneumoniae isolates. Phages SU503 and SU552A show high pairwise nucleotide identity to Klebsiella phages KP34 (NC_013649), F19 (NC_023567) and NTUH-K2044-K1-1 (NC_025418). Bioinformatic analysis of these phage genomes show high conservation of gene arrangement and gene content, conserved catalytically active residues of their RNA polymerase, a common and specific lysis cassette, and form a joint cluster in phylogenetic analysis of their conserved genes. Also, we have performed biological characterization of the burst size, latent period, host specificity (together with KP34 and NTUH-K2044-K1-1), morphology, and structural genes as well as sensitivity testing to various conditions. Based on the analyses of these phages, the creation of a new phage genus is suggested within the Autographivirinae, called “Kp34likevirus” after their type phage, KP34. This genus should encompass the recently genome sequenced Klebsiella phages KP34, SU503, SU552A, F19 and NTUH-K2044-K1-1.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.