Production of β-Maltooligosaccharides of α- and δ-Tocopherols by Klebsiella pneumoniae and Cyclodextrin Glucanotransferase as Anti-allergic Agents

Molecules

Kei Shimoda²  Masaaki Akagi¹ 

[1] Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan;Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan

关键词: tocopherol;    Klebsiella pneumoniae;    cyclodextrin glucanotransferase;    β-maltooligosaccharide;    anti-allergic activity;   

DOI  :  10.3390/molecules14083106

来源: mdpi

【 摘 要 】

The glycosylation of α- and δ-tocopherols using Klebsiella pneumoniae and cyclodextrin glucanotransferase (CGTase) was investigated. K. pneumoniae converted α- and δ-tocopherols into the corresponding β-glucosides in 10 and 8% yield, respectively. CGTase glycosylated α-tocopheryl β-glucoside to α-tocopheryl β-maltoside (51%) and α-tocopheryl β-maltotrioside (35%). On the other hand, δ-tocopheryl β-glucoside was converted into the corresponding β-maltoside (45%) and β-maltotrioside (29%) by CGTase. The β-glucoside of α-tocopherol, and β-glucoside and β-maltoside of δ-tocopherol showed inhibitory effects on IgE antibody production and on histamine release from rat peritoneal mast cells.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.