miR-182-5p Regulates Nogo-A Expression and Promotes Neurite Outgrowth of Hippocampal Neurons In Vitro

Pharmaceuticals

Rodrigo M. Maza¹  Altea Soto¹  David Reigada¹  María Asunción Barreda-Manso¹  Manuel Nieto-Díaz¹  Teresa Muñoz-Galdeano¹ 

[1] Molecular Neuroprotection Group, Research Unit, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain;

关键词: miR-182-5p;    Nogo-A;    neurite outgrowth;    axonal regeneration;    spinal cord injury;    neurodegenerative diseases;   

DOI  :  10.3390/ph15050529

来源: DOAJ

【 摘 要 】

Nogo-A protein is a key myelin-associated inhibitor of axonal growth, regeneration, and plasticity in the central nervous system (CNS). Regulation of the Nogo-A/NgR1 pathway facilitates functional recovery and neural repair after spinal cord trauma and ischemic stroke. MicroRNAs are described as effective tools for the regulation of important processes in the CNS, such as neuronal differentiation, neuritogenesis, and plasticity. Our results show that miR-182-5p mimic specifically downregulates the expression of the luciferase reporter gene fused to the mouse Nogo-A 3′UTR, and Nogo-A protein expression in Neuro-2a and C6 cells. Finally, we observed that when rat primary hippocampal neurons are co-cultured with C6 cells transfected with miR-182-5p mimic, there is a promotion of the outgrowth of neuronal neurites in length. From all these data, we suggest that miR-182-5p may be a potential therapeutic tool for the promotion of axonal regeneration in different diseases of the CNS.

【 授权许可】

Unknown