Djhsp60 Is Required for Planarian Regeneration and Homeostasis

Biomolecules

Gege Song¹  Fangying Guo²  Guangwen Chen²  Rui Li²  Meng Wu²  Xinwei Li³  Kexue Ma³  Qiong Lu³ 

[1] Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Department of Basic Medicine, Luohe Medical College, Luohe 462002, China;

关键词: planarian;    regeneration;    HSP60;    mitochondria;    cathepsin L;   

DOI  :  10.3390/biom12060808

来源: DOAJ

【 摘 要 】

HSP60, a well-known mitochondrial chaperone, is essential for mitochondrial homeostasis. HSP60 deficiency causes dysfunction of the mitochondria and is lethal to animal survival. Here, we used freshwater planarian as a model system to investigate and uncover the roles of HSP60 in tissue regeneration and homeostasis. HSP60 protein is present in all types of cells in planarians, but it is relatively rich in stem cells and head neural cells. Knockdown of HSP60 by RNAi causes head regression and the loss of regenerating abilities, which is related to decrease in mitotic cells and inhibition of stem cell-related genes. RNAi-HSP60 disrupts the structure of the mitochondria and inhibits the mitochondrial-related genes, which mainly occur in intestinal tissues. RNAi-HSP60 also damages the integrity of intestinal tissues and downregulates intestine-expressed genes. More interestingly, RNAi-HSP60 upregulates the expression of the cathepsin L-like gene, which may be the reason for head regression and necrotic-like cell death. Taking these points together, we propose a model illustrating the relationship between neoblasts and intestinal cells, and also highlight the essential role of the intestinal system in planarian regeneration and tissue homeostasis.

【 授权许可】

Unknown