Discovery of potential ovarian cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

OpuholiŽenskoj Reproduktivnoj Sistemy

E. V. Ogorodnikova¹  N. R. Pogosyan²  K. I. Zhordania²  A. N. Gritsay²  N. E. Arnotskaya³  V. E. Shevchenko³ 

[1] Blood Transfusion Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow;Gynecology Department, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow;Oncoproteomics Laboratory, N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow;

关键词: biomarkers;    ovarian cancer;    proteome;    blood plasma;    mass spectrometry;   

DOI  :  10.17650/1994-4098-2013-0-3-4-90-96

来源: DOAJ

【 摘 要 】

At present, there is no screening test for the early detecting and monitoring of ovarian cancer, one of the most lethal form of gynaecological malig- nancy in the worldwide. In this study, the new methodology for the search of tumor markers of ovarian cancer, involving profiling the low-molec- ular blood plasma proteomes, is developed, unified and approved. The given approach included three basic components: pre-preparation of samples, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics software for mass spectral data process- ing. Opportunities and prospects of the developed approach for the detection of potential ovarian cancer markers were shown. For search of po- tential tumor markers, screening of 40 blood plasma samples from ovarian cancer patients and 48 control samples were carried out. As a result of the present research, peptides/polypeptides which can be used in future for detecting this pathology were found out.

【 授权许可】

Unknown