Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model

Genetics and Molecular Biology

Wen-Chih Wu¹  Tsan Yang²  Chien-An Sun³  Jen-Chun Kuan³  Jih-Fu Hsieh³  Yu-Ching Chou³  Chih-Hsiung Hsu³  Chang-Chieh Wu⁴ 

[1] Kaohsiung Armed Forces General Hospital;Meiho University;National Defense Medical Center;Tri-Service General Hospital;

关键词: colorectal cancer;    DNA methylation;    stool test for colorectal cancer;   

DOI  :  10.1590/S1415-47572013000300004

来源: DOAJ

【 摘 要 】

A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening.

【 授权许可】

Unknown