Effectiveness of Low-Cost Bioreactors Integrated with a Gas Stripping System for Butanol Fermentation from Sugarcane Molasses by Clostridium beijerinckii

Fermentation

Likit Sirisantimethakom¹  Lakkana Laopaiboon²  Pattana Laopaiboon²  Thanawat Thanapornsin³ 

[1] Department of Biotechnology, Faculty of Agro-Industrial Technology, Kalasin University, Kalasin 46000, Thailand;Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand;Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand;

关键词: renewable energy;    butanol production;    sugarcane molasses;    Clostridium sp.;    bubble column bioreactor;    gas-lift column bioreactor;   

DOI  :  10.3390/fermentation8050214

来源: DOAJ

【 摘 要 】

The effectiveness of column bioreactors for butanol fermentation from sugarcane molasses by Clostridium beijerinckii TISTR 1461 was investigated. This fermentation was operated at an initial pH of 6.5 and temperature of 37 °C under anaerobic conditions. A 1-L bubble column bioreactor was used with various gas circulation rates ranging from 0.2 to 1.0 L/min. The highest butanol concentration (P_B, 8.72 g/L), productivity (Q_B, 0.24 g/L∙h) and yield (Y_B/S, 0.21 g/g) were obtained with a gas circulation of 0.2 L/min. To improve butanol production efficiency, gas-lift column bioreactors with internal and external loops at 0.2 L/min of circulating gas were used. Higher P_B (10.50–10.58 g/L), Q_B (0.29 g/L∙h) and Y_B/S (0.22–0.23 g/g) values were obtained in gas-lift column bioreactors. These values were similar to those using a more complex 2-L stirred-tank bioreactor (P_B, 10.10 g/L; Q_B, 0.28 g/L h and Y_B/S, 0.22 g/g). Hence, gas-lift column bioreactors have potential for use as low-cost fermenters instead of stirred-tank bioreactors for butanol fermentation. When the gas-lift column bioreactor with an internal loop was coupled with a gas stripping system, it yielded an enhanced P_B and sugar consumption of approximately 9% and 7%, respectively, compared to a system with no gas stripping.

【 授权许可】

Unknown