Sequence analysis of bla CTX-M-28 , an ESBL responsible for third-generation cephalosporin resistance in Enterobacteriaceae, for the first time in India

Indian Journal of Pathology and Microbiology

关键词: β;    -lactamases;    bla _CTX-M-28;    Enterobacteriaceae;    ESBL;    point mutation;   

DOI  :  

来源: DOAJ

【 摘 要 】

The most common group of ESBLs not belonging to the bla _TEMor bla _SHVfamilies were termed bla _CTX-M , to highlight their ESBLs′ greater activity against cefotaxime than against ceftazidime. The presence of nosocomial bla _CTX-M-28 -producing Enterobacteriaceae strains has not been reported earlier in Indian hospitals. The sequences of bla _CTX-M-28gene from cephalosporin-resistant Enterobacteriaceae were analyzed. The structural gene encodes a 290 amino-acid protein, which is most related to the bla _CTX-Mβ-lactamases. The conserved K-T-G was identified in the bla _CTX-M-28protein sequence, but significantly, two point mutations (N→T) and (F→S) were identified in the Y-G-N- and S-T-F-K-conserved motifs respectively. These point mutations were seen in all the three sequenced isolates

【 授权许可】

Unknown