Molecules | |
A New 9,10-Dihydrophenanthrene and Cell Proliferative3,4-δ-Dehydrotocopherols from Stemona tuberosa | |
Hyun Ae Woo1  Eun-Kyoung Seo1  Yun-Seo Kil1  Jiyoung Park1  Ah-Reum Han1  | |
[1] College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University,Seoul 120-750, Korea; | |
关键词: Stemona tuberosa; Stemonaceae; 9,10-dihydrophenanthrene; 3,4-δ-dehydrotocopherol; chiral separation; cell proliferation; | |
DOI : 10.3390/molecules20045965 | |
来源: DOAJ |
【 摘 要 】
A new compound, 9,10-dihydro-5-methoxy-8-methyl-2,7-phenanthrenediol (1), was isolated from the roots of Stemona tuberosa Lour. (Stemonaceae) together withtwo new optically active compounds, (2S,4'R,8'R)-3,4-δ-dehydrotocopherol (2) and (2R,4'R,8'R)-3,4-δ-dehydrotocopherol (3). The structures of compounds 1–3 were determined on the basis of spectroscopic data analysis. Compounds 2 and 3 were each purified from a stereoisomeric mixture of 2 and 3 by preparative HPLC using a chiral column for the first time. The absolute configurations at C-2 of 2 and 3 were determined by Circular Dichroism (CD) experiments. As a part of the research to find natural wound healing agents, all isolates and the mixture of 2 and 3 were evaluated for their cell proliferative effects using a mouse fibroblast NIH3T3 and a HeLa human cervical cancer cell line. As a result, 1, 2, 3, or the mixture of 2 and 3 showed 41.6%, 78.4%, 118.6%, 38.2% increases of cell proliferation in the mouse fibroblast NIH3T3 respectively, compared to 28.4% increase of δ-tocopherol. Moreover, none of them induced cancer cell proliferation. Therefore, 3,4-δ-dehydrotocopherols, especially pure isomers 2 and 3 can be suggested as potential wound healing agents.
【 授权许可】
Unknown