Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art

Cell Regeneration

Eleni Oberbauer¹  Christian Gabriel¹  Susanne Wolbank¹  Heinz Redl¹  Carolin Steffenhagen¹  Christoph Wurzer¹ 

[1] Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, Austria;

关键词: Human adipose tissue;    Stromal vascular fraction;    Adipose-derived stromal/stem cells;    Enzymatic;    Non-enzymatic;    Isolation systems;   

DOI  :  10.1186/s13619-015-0020-0

来源: DOAJ

【 摘 要 】

In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell population including the adipose-derived stromal/stem cells (ASC), which showed regenerative potential in several clinical studies and trials. SVF should be provided in a safe and reproducible manner in accordance with current good manufacturing practices (cGMP). To ensure highest possible safety for patients, a precisely defined procedure with a high-quality control is required. Hence, an increasing number of adipose tissue-derived cell isolation systems have been developed. These systems aim for a closed, sterile, and safe isolation process limiting donor variations, risk for contaminations, and unpredictability of the cell material. To isolate SVF from adipose tissue, enzymes such as collagenase are used. Alternatively, in order to avoid enzymes, isolation systems using physical forces are available. Here, we provide an overview of known existing enzymatic and non-enzymatic adipose tissue-derived cell isolation systems, which are patented, published, or already on the market.

【 授权许可】

Unknown