Prevalence of drug-resistant microbes in sepsis cases of catheter and fistula based haemodialysis

Saudi Journal of Biological Sciences

M. Ijaz Khan¹  Mater H. Mahnashi¹  Salim Al-Rejaie¹  Irshad Muhammad²  Osama M Alshehri³  Dawood Ahmed⁴  Habiba Daud⁵  Waqas Ahmed⁵  Rui Jiang⁶  Muhammad Awais⁷  Mohammed M Jalal⁸ 

[1] Corresponding authors.;Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Sialkot, Punjab, Pakistan;Department of Mathematics and Statistics, Riphah International University I-14, Islamabad 44000, Pakistan;Department of Medical Lab Technology, The University of Haripur, Haripur, Pakistan;Department of Microbiology, The University of Haripur, Haripur, Pakistan;Department of Nephrology, Huzhou Central Hospital, Zhejiang, Huzhou 313003, PR China;Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;School of Chemical Engineering, Sungyunkwan University, Suwon, South Korea;

关键词: Drug resistance;    Sepsis;    Catheter;    Fistula;    Hemodialysis;    ESRD;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Chronic stage renal disease is a severe disease of the kidney which affects people globally. According to the global burden of diseases in 2010, this disease has caused more deaths worldwide and due to the high death rate, the ESRD (end-stage renal disease) is now ranked up from 27th to 18th range in the list. Methodology: Dialysis samples were collected from the Haripur city and surrounding areas. Samples were inoculated on different selective media for bacterial growth. In addition, different biochemical tests were also performed for identification, where as the resistance genes were identified through a polymerase chain reaction. Result: Out of the total 100 dialysis patient’s blood samples, only 17 showed the presence of gram-positive bacteria i.e., Staphylococcus aureus while two shown the presence of gram-negative bacteria i.e., Klebsiella pneumoniaeee and Pseudomonas aeruginosa. While in molecular identification two antibiotic resistance genes muc and mecA belong to the staphylococcus strain shown their presence. Conclusion: A high infection rate has been observed in fistula-based hemodialysis (17(77.27%)) as compares to catheter-based hemodialysis (5(22.3%) with no significant difference of incidence between the groups (p > 0.05).

【 授权许可】

Unknown