Genes | |
The Genome of the Steller Sea Lion (Eumetopias jubatus) | |
Ryan Tan1  HarwoodH. Kwan1  MarcoA. Marra1  AndrewJ. Mungall1  ShaunD. Jackman1  Tina MacLeod1  Heather Kirk1  Inanc Birol1  Eric Chuah1  Sreeja Leelakumari1  Kane Tse1  Dean Cheng1  StevenJ. M. Jones1  Yongjun Zhao1  Rebecca Carlsen1  Richard Moore1  Luka Culibrk1  GregoryA. Taylor1  Pawan Pandoh1  DavidA.S. Rosen2  Martin Haulena3  | |
[1] Canada’s Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada;Institute for the Oceans and Fisheries, University of British Columbia, Vancouver, BC V6T-1Z4, Canada;Vancouver Aquarium, Vancouver, BC V6G 3E2, Canada; | |
关键词: Steller sea lion; Eumetopias jubatus; genome; microfluidic partitioning; nanopore; marine animal; | |
DOI : 10.3390/genes10070486 | |
来源: DOAJ |
【 摘 要 】
The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
【 授权许可】
Unknown