Immunohistochemical Localization of Steroidogenic Enzymes in the Corpus Luteum and the Placenta of the Ribbon Seal (Phoca fasciata) and Steller Sea Lion (Eumetopias jubatus)

Journal of Veterinary Medical Science

Masatsugu SUZUKI³  J. Ian MASON²  Yukie YAMAMOTO¹  Nobuhiro HARADA⁴  Noriyuki OHTAISHI³  Takeomi ISONO¹  Mitsuru WATABE⁵  Masatoshi TSUNOKAWA⁵  Tsuyoshi ISHINAZAKA³ 

[1] Research Institute of North Pacific Fisheries, Hokkaido University;Royal Infirmary of Edinburgh NHS Trust;Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University;Department of Biochemistry, Fujita Health University School of Medicine;Otaru Aquarium

关键词: corpus luteum;    placenta;    ribbon seal;    Steller sea lion;    steroidogenic enzyme;   

DOI  :  10.1292/jvms.63.955

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(9)To study the luteal and placental function of pinnipeds, we analyzed the localization of steroidogenic enzymes (P450scc, 3 β HSD and P450arom) in the corpus luteum and the placenta of ribbon seals (Phoca fasciata) and Steller sea lions (Eumetopias jubatus) immunohistochemically. P450scc and 3 β HSD were present in all luteal cells of both species. Almost all of the luteal cells were immunostained for P450arom, while P450scc and 3 β HSD were negatively immunostained in placentae and P450arom was present in the syncytiotrophoblast of placentae. These findings suggest that 1) corpora lutea of both species synthesize pregnenolone, progesterone and estrogen during the entire pregnancy period, and 2) like other terrestrial carnivores in the suborder Caniformia, placentae of both species do not have the capability for synthesizing progesterone in the latter half of active pregnancy period.

【 授权许可】

Unknown