Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

BioDiscovery

关键词: ELF-EMF;    primary lymphocytes;    apoptosis;    DNA damag;   

DOI  :  10.7750/BioDiscovery.2012.2.2

来源: DOAJ

【 摘 要 】

The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p

【 授权许可】

Unknown