Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Journal of Lasers in Medical Sciences

Hadi Hasanzadeh¹  Samaneh Seyyedi²  Hakimeh Zali³  Mostafa Rezaie-Tavirani⁴ 

[1] Cancer Research Center and Department of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran;Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran;Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

关键词: ELF-EMF;    Proteomics;    Neuroblastoma;    Protein expression;    intensity;   

DOI  :  10.22037/2010.v8i2.12448

学科分类：基础医学

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

  Introduction:During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields (ELF-EMF) emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study. Methods:SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz (ELF-EMF) for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software. Results:Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced. Conclusion:Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation.

【 授权许可】

CC BY