Gut Bacterial Flora of Open Nested Honeybee, Apis florea

Frontiers in Ecology and Evolution

Samy Sayed¹  Showket A. Dar²  Mustafa Shukry³  D. N. Ganeshprasad⁴  Jafar K. Lone⁴  A. H. Sneharani⁴  Muntazir Mushtaq⁵  Yogesh S. Shouche⁶  Kunal Jani⁶  Khalid Ali Khan⁷ 

[1] Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt;Department of Entomology, Krishi Vigyan Kendra (KVK)-Srinagar, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, India;Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh, Egypt;Department of Studies in Biochemistry, Jnana Kaveri Post-Graduate Centre, Mangalore University, Chikka Aluvara, Kodagu, India;Indian Council of Agricultural Research (ICAR)-National Bureau of Plant Genetic Resources, Division of Germplasm Evaluation, New Delhi, India;National Centre for Microbial Resource, National Centre for Cell Science, Pune, India;Unit of Bee Research and Honey Production, Research Center for Advanced Materials Science (RCAMS), Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia;

关键词: dwarf honeybee;    Apis florea;    gut microbiota;    MALDI-TOF MS;    16S rRNA;    next-generation sequencing;   

DOI  :  10.3389/fevo.2022.837381

来源: DOAJ

【 摘 要 】

Honeybees are eusocial insects with close interaction with their surrounding environment. Gut microbiota in honeybees play a significant role in host health, biology, and interaction behavior with the surrounding environment. Apis florea, a wild bee, is the most primitive among all honeybees and is indigenous to the Indian subcontinent. Previous reports on reared honeybee species provide information on the gut microbiome. No such studies are reported on the gut microbiota of the wild honeybee species. This study aimed at studying the gut microbiome of the wild honeybee species, A. florea. The study reports the analysis and the identification of gut bacteria in the wild honeybee species, A. florea, employing culture-based and culture-independent methods. Cultured bacteria were identified and characterized by MALDI-TOF MS and 16S rRNA sequencing. A comprehensive analysis and identification of non-culturable bacteria were performed by 16S rRNA amplicon next-generation sequencing. This approach splits gut bacteria into four bacterial phyla, four families, and 10 genera in major. The dominant taxa identified in A. florea belonged to the family Enterobacteriaceae (79.47%), Lactobacillaceae (12.75%), Oxalobacteraceae (7.45%), and Nocardiaceae (0.13%). The prevailing bacteria belonged to Enterobacter, Lactobacillus, Escherichia-Shigella, Massilia, Klebsiella, Citrobacter, Pantoea, Serratia, Rhodococcus, and Morganella genera, belonging to phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. This study observed the occurrence of a few bacteria that are not previously reported for their occurrence in other species of the Apis genus, making this investigation highly relevant with regard to the bee microbiome.

【 授权许可】

Unknown