Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses

Drug Delivery

James McCoy¹  Samuel Amoah²  Shivaprakash Gangappa²  Weiping Cao²  Caitlin Bohannon²  Suryaprakash Sambhara²  Margarita Mishina²  Suresh K. Mittal³  Wadzanai P. Mboko³ 

[1] Emory University;National Center for Immunization and Respiratory Diseases;Purdue University;

关键词: cationic polymers;    h5n1 vaccine;    adaptive immunity;    protective immunity;    mouse model;    influenza;   

DOI  :  10.1080/10717544.2018.1450909

来源: DOAJ

【 摘 要 】

Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet™ or in vivo-jetPEI®, and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed TH1/TH2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines.

【 授权许可】

Unknown