Probing Immune-Mediated Clearance of Acute Middle Ear Infection in Mice

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Longhuan Ma¹  Amanda D. Caulfield¹  Kalyan K. Dewan¹  Yang Su¹  Colleen Sedney¹  Eric T. Harvill¹  Uriel Blas-Machado² 

[1] Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States;Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, United States;

关键词: otitis media;    Bordetella bronchiseptica;    natural infection;    adaptive immunity;    protective immunity;   

DOI  :  10.3389/fcimb.2021.815627

 received in 2021-11-15, accepted in 2021-12-21,  发布年份 2022

来源: Frontiers

【 摘 要 】

Acute otitis media (AOM) is commonly caused by bacterial pathobionts of the nasopharynx that ascend the Eustachian tube to cause disease in the middle ears. To model and study the various complexities of AOM, common human otopathogens are injected directly into the middle ear bullae of rodents or are delivered with viral co-infections which contribute to the access to the middle ears in complex and partially understood ways. Here, we present the novel observation that Bordetella bronchiseptica, a well-characterized respiratory commensal/pathogen of mice, also efficiently ascends their Eustachian tubes to colonize their middle ears, providing a flexible mouse model to study naturally occurring AOM. Mice lacking T and/or B cells failed to resolve infections, highlighting the cooperative role of both in clearing middle ear infection. Adoptively transferred antibodies provided complete protection to the lungs but only partially protected the middle ears, highlighting the differences between respiratory and otoimmunology. We present this as a novel experimental system that can capitalize on the strengths of the mouse model to dissect the molecular mechanisms involved in the generation and function of immunity within the middle ear.

【 授权许可】

Unknown   
Copyright © 2022 Dewan, Sedney, Caulfield, Su, Ma, Blas-Machado and Harvill