期刊论文详细信息
Frontiers in Pharmacology
Antioxidant Phytoconstituents From Onosma bracteata Wall. (Boraginaceae) Ameliorate the CCl4 Induced Hepatic Damage: In Vivo Study in Male Wistar Rats
Varinder Kaur1  Katrin Sak1  Subheet Kumar Jain2  Ajay Kumar3  Hardeep Singh Tuli3  Kritika Pandit4  Satwinderjeet Kaur5 
[1] Environmental Sciences, Guru Nanak Dev University, Amritsar, India;Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Ambala, India;;Department of Botanical &Indigenous Education and Research Centre, James Cook University, Townsville, QLD, Australia;NGO, Praeventio, Tartu, Estonia;
关键词: antimutagenicity;    apoptosis;    carcinogenesis;    hepatoprotective effect;    Onosma bracteata;    phytoconstituents;   
DOI  :  10.3389/fphar.2020.01301
来源: DOAJ
【 摘 要 】

Onosma bracteata Wall. (Boraginaceae) is a highly valuable medicinal herb that is used for the treatment of fever, bronchitis, asthma, rheumatism, stomach irritation, and other inflammatory disorders. The present study aims to explore the hepatoprotective potential of ethanolic extract (Obeth) from O. bracteata aerial parts against carbon tetrachloride (CCl4) which causes hepatic damage in the male Wistar rats. Obeth showed effective radical quenching activity with an EC50 of 115.14 and 199.33 µg/mL in superoxide radical scavenging and lipid peroxidation analyses respectively along with plasmid DNA protective potential in plasmid nicking assay. The Obeth modulated mutagenicity of 2 Aminofluorine (2AF) in the pre-incubation mode of investigation (EC50 10.48 µg/0.1 mL/plate) in TA100 strain of Salmonella typhimurium. In in vivo studies, pretreatment of Obeth (50, 100, and 200 mg/kg) had the potential to normalize the biochemical markers aggravated by CCl4 (1mL/kg b.wt.) including liver antioxidative enzymes. Histopathological analysis also revealed the restoration of CCl4-induced liver histopathological alterations. Immunohistochemical studies showed that the treatment of Obeth downregulated the expression levels of p53 and cyclin D in hepatocytes. and downregulation in the Western blotting analysis revealed the downregulation of p-NF-kB, COX-2, and p53. HPLC data analysis showed the supremacy of major compounds namely, catechin, kaempferol, epicatechin, and Onosmin A in Obeth. The present investigation establishes the hepatoprotective and chemopreventive potential of O. bracteata against CCl4-induced hepatotoxicity via antioxidant defense system and modulation of the expression of proteins associated with the process of carcinogenesis in hepatic cells.

【 授权许可】

Unknown   

  文献评价指标  
  下载次数:0次 浏览次数:1次