Sesamin Enhances Cholesterol Efflux inRAW264.7 Macrophages

Molecules

Nan Liu¹  Lizhong Sun²  Jun Zheng²  Chongming Wu³  Peng Guo³ 

[1] Cardiac Surgical Intensive Care Unit, Beijing Anzhen Hospital, Capital Medical University,Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China;Department of Cardiovascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China;Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, China;

关键词: sesamin;    atherosclerosis;    foam cell;    cholesterol efflux;    macrophage;   

DOI  :  10.3390/molecules19067516

来源: DOAJ

【 摘 要 】

Foam cells formation as a result of the uncontrolled cytophagy of modified cholesterol by macrophages plays a key role in the occurrence and development of atherosclerosis. Sesamin is an active constituent of Sesamum indicum which has been shown to possess multiple pharmacological activities. In this work, we investigated the effects of sesamin on foam cell formation and cholesterol efflux in RAW264.7 macrophages. Sesamin dose-dependently inhibited the enhanced cholesterol accumulation elicited by oxidized low-density lipoprotein cholesterol (oxLDL) in RAW264.7 cells. Treatment with sesamin (10 μM) significantly enhanced cholesterol efflux mediated by high-density lipoprotein (HDL). Realtime quantitative PCR and luciferase assays showed that sesamin significantly increased the mRNA levels of PPARγ, LXRα, and ABCG1, and increased the transcriptional activity of PPARγ. The stimulating effect of sesamin on cholesterol efflux was substantially inhibited by the co-treatment with GW9662,a potent inhibitor of PPARγ. These results suggest that sesamin is a new inhibitor of foam cell formation that may stimulate cholesterol efflux through upregulation of thePPARγ-LXRα-ABCG1 pathway.

【 授权许可】

Unknown