Application of Adaptive Neuro-Fuzzy Inference System-Non-dominated Sorting Genetic Algorithm-II (ANFIS-NSGAII) for Modeling and Optimizing Somatic Embryogenesis of Chrysanthemum

Frontiers in Plant Science

Mohsen Hesami¹  Roohangiz Naderi¹  Mohsen Yoosefzadeh-Najafabadi²  Masoud Tohidfar³ 

[1] Department of Horticultural Science, Faculty of Agriculture, University of Tehran, Karaj, Iran;Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada;Department of Plant Biotechnology, Faculty of Science and Biotechnology, Shahid Beheshti University, Tehran, Iran;

关键词: artificial intelligence;    chrysanthemum;    carbohydrate;    embryogenesis;    in vitro culture;    light quality;   

DOI  :  10.3389/fpls.2019.00869

来源: DOAJ

【 摘 要 】

A hybrid artificial intelligence model and optimization algorithm could be a powerful approach for modeling and optimizing plant tissue culture procedures. The aim of this study was introducing an Adaptive Neuro-Fuzzy Inference System- Non-dominated Sorting Genetic Algorithm-II (ANFIS-NSGAII) as a powerful computational methodology for somatic embryogenesis of chrysanthemum, as a case study. ANFIS was used for modeling three outputs including callogenesis frequency (CF), embryogenesis frequency (EF), and the number of somatic embryo (NSE) based on different variables including 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), sucrose, glucose, fructose, and light quality. Subsequently, models were linked to NSGAII for optimizing the process, and the importance of each input was evaluated by sensitivity analysis. Results showed that all of the R2 of training and testing sets were over 92%, indicating the efficiency and accuracy of ANFIS on the modeling of the embryogenesis. Also, according to ANFIS-NSGAII, optimal EF (99.1%), and NSE (13.1) can be obtained from a medium containing 1.53 mg/L 2,4-D, 1.67 mg/L BAP, 13.74 g/L sucrose, 57.20 g/L glucose, and 0.39 g/L fructose under red light. The results of the sensitivity analysis showed that embryogenesis was more sensitive to 2,4-D, and less sensitive to fructose. Generally, the hybrid ANFIS-NSGAII can be recognized as a powerful computational tool for modeling and optimizing in plant tissue culture.

【 授权许可】

Unknown