First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

Pathogens

Gustavo Fontecha¹  Osman Archaga¹  Sergio Betancourth¹  Wendy Moncada²  Vilma Rodríguez² 

[1] Microbiology Research Institute, Universidad Nacional Autónoma de Honduras, Tegucigalpa 11101, Honduras;Servicio de Atención Integral para Pacientes que Viven con VIH/SIDA-Instituto Nacional Cardiopulmonar, Tegucigalpa 11101, Honduras;

关键词: Cryptosporidium spp;    C. parvum;    gp60;    18S ribosomal;    cowp;    HIV/AIDS;   

DOI  :  10.3390/pathogens10030336

来源: DOAJ

【 摘 要 】

Cryptosporidiosis is one of the most important causes of gastroenteritis in the world, especially in low- and middle-income countries. It is caused by the Apicomplexan parasite Cryptosporidium spp., and mainly affects children and immunocompromised people, in whom it can pose a serious threat to their health, or even be life threatening. In Honduras, there are no data on parasite species or on molecular diversity or Cryptosporidium subtypes. Therefore, a cross-sectional study was conducted between September 2019 and March 2020 for the molecular identification of Cryptosporidium spp. in 102 patients living with HIV who attended a national hospital in Tegucigalpa. Stool samples were analyzed by direct microscopy, acid-fast stained smears, and a rapid lateral flow immunochromatographic test. All samples that tested positive were molecularly analyzed to identify the species and subtype of the parasite using three different markers: gp60, cowp, and 18Sr. PCR products were also sequenced. Four out of 102 samples (3.92%) were positive for Cryptosporidiumparvum, and all were assigned to subtype IIa. These findings suggest a possible zoonotic transmission in this population.

【 授权许可】

Unknown