Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe

Microorganisms

Martin Kváč¹  Evi Canniere²  Hélène Leruste³  Pedro Pinto⁴  Cláudia A. Ribeiro⁴  Sumaiya Hoque⁴  Anastasios D. Tsaousis⁴  Anne Barbier Bourgeois⁵  Martine Dellevoet⁶  Janine Roemen⁶  Yvonne Daandels⁶  Ourida Hammouma⁷  Jérôme Follet⁸  Sébastien Détriché⁹ 

[1] Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Parasitology, 37005 České Budějovice, Czech Republic;Inagro vzw, Ieperseweg 87, 8800 Rumbeke-Beitem, Belgium;Junia, Comportement Animal et Systèmes d’Elevage, F-59000 Lille, France;Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NZ, UK;Selas, 31 Rue de la République, 59496 Salome, France;Southern Agricultural and Horticultural Organisation (ZLTO), Onderwijsboulevard 225, 5223 DE’s-Hertogenbosch, The Netherlands;UMR-Transfrontalière 1158 BioEcoAgro, Junia, University of Lille, University of Liège, UPJV, ULCO, University of Artois, INRAE, F-59000 Lille, France;University of Lille, CNRS, Centrale Lille, Junia, University Polytechnique Hauts de France, UMR 8520 IEMN Institut d’Electronique de Microélectronique et de Nanotechnologie, F 59000 Lille, France;University of Lille, Institut Mines-Télécom, University of Artois, Junia, ULR 4515—LGCgE, Laboratoire de Génie Civil et Géo-Environnement, F-59000 Lille, France;

关键词: 18S rRNA;    Cryptosporidium;    dairy cattle;    gp60;    genotyping;    prevalence;   

DOI  :  10.3390/microorganisms9112394

来源: DOAJ

【 摘 要 】

Cryptosporidium is an apicomplexan parasitic protist, which infects a wide range of hosts, causing cryptosporidiosis disease. In farms, the incidence of this disease is high in animals such as cows, leading to extensive economic loss in the livestock industry. Infected cows may also act as a major reservoir of Cryptosporidium spp., in particular C. parvum, the most common cause of cryptosporidiosis in these animals. This poses a risk to the trading of livestock, to other farms via breeding centres, and to human health. This study is a part of a global project aimed at strategies to tackle cryptosporidiosis. To reach this target, it was essential to determine whether prevalence was dependent on the studied countries or if the issue was borderless. Indeed, C. parvum occurrence was assessed across dairy farms in certain regions of Belgium, France, and the Netherlands. At the same time, the animal-to-animal transmission of the circulating C. parvum subtypes was studied. To accomplish this, we analysed 1084 faecal samples, corresponding to 57 dairy farms from all three countries. To this end, 18S rRNA and gp60 genes fragments were amplified, followed by DNA sequencing, which was subsequently used for detection and subtyping C. parvum. Bioinformatic and phylogenetic methods were integrated to analyse and characterise the obtained DNA sequences. Our results show 25.7%, 24.9% and 20.8% prevalence of Cryptosporidium spp. in Belgium, France, and the Netherlands respectively. Overall, 93% of the farms were Cryptosporidium positive. The gp60 subtyping demonstrated a significant number of the C. parvum positives belonged to the IIa allelic family, which has been also identified in humans. Therefore, this study highlights how prevalent C. parvum is in dairy farms and further suggests cattle as a possible carrier of zoonotic C. parvum subtypes, which could pose a threat to human health.

【 授权许可】

Unknown