Molecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques

Frontiers in Plant Science

Rafael Tavares¹  Lucia Mattiello²  Marcelo Menossi²  Mark Rütgers²  Maria Fernanda Sua-Rojas²  José Sérgio Soares²  Kevin Begcy³ 

[1] Cell and Developmental Biology, John Innes Centre, Norwich, United Kingdom;Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil;Environmental Horticulture Department, University of Florida, Gainesville, FL, United States;

关键词: CRISPR/Cas9;    base editing;    prime editing;    genome editing efficiency;    DNA-free CRISPR/Cas;   

DOI  :  10.3389/fpls.2022.868027

来源: DOAJ

【 摘 要 】

The prokaryote-derived Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas mediated gene editing tools have revolutionized our ability to precisely manipulate specific genome sequences in plants and animals. The simplicity, precision, affordability, and robustness of this technology have allowed a myriad of genomes from a diverse group of plant species to be successfully edited. Even though CRISPR/Cas, base editing, and prime editing technologies have been rapidly adopted and implemented in plants, their editing efficiency rate and specificity varies greatly. In this review, we provide a critical overview of the recent advances in CRISPR/Cas9-derived technologies and their implications on enhancing editing efficiency. We highlight the major efforts of engineering Cas9, Cas12a, Cas12b, and Cas12f proteins aiming to improve their efficiencies. We also provide a perspective on the global future of agriculturally based products using DNA-free CRISPR/Cas techniques. The improvement of CRISPR-based technologies efficiency will enable the implementation of genome editing tools in a variety of crop plants, as well as accelerate progress in basic research and molecular breeding.

【 授权许可】

Unknown