Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

Frontiers in Genetics

Genetics

Danilo Maddalo¹  Mirunalini Ravichandran² 

[1] null;[e-mail];

关键词: CRISPR/Cas9;    CRISPRa/i;    precision oncology;    epigenome editing;    base editing;    prime editing;    cancer modeling;    CRISPR screening;   

DOI  :  10.3389/fgene.2023.1273994

 received in 2023-08-07, accepted in 2023-09-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery.

【 授权许可】

Unknown   
Copyright © 2023 Ravichandran and Maddalo.