Allium test - some questions

Acta Societatis Botanicorum Poloniae

Danuta Antosiewicz¹  Małgorzata Wierzbicka¹ 

[1] University of Warsaw;

关键词: Allium cepa L.;    Allium test;    mitotic activity;    root tip;    lead;    lead in apoplast;    variation in test material;   

DOI  :  10.5586/asbp.1988.020

来源: DOAJ

【 摘 要 】

The paper concerns some problems connected with the experiments with the use of the Allium test. The variation degree of the material analysed is also presented. For the sake of standard experiments using the Allium test, it is suggested to grow onion bulbs in deionized water or in Hoagland nutrient. Taking as an example the lead compounds, the paper proves that the kind of water which has been used to prepare the analyzed solution affects significantly the toxicity level of the tested compound. This may be the reason why the results of experiments made by various researchers are incomparable. The differences in metabolism between individual roots are studied as well, both in the control and the exprimental materials. Eventually, it is suggested that the roots of each bulb should be treated individually in all Allium test experiments. The presented approach largely increases accuracy and repeatability of the Allium test results.

【 授权许可】

Unknown