Wild Relatives of Wheat Respond Well to Water Deficit Stress: A Comparative Study of Antioxidant Enzyme Activities and Their Encoding Gene Expression

Agriculture

Peter Poczai¹  MohammadReza Naghavi²  Mansoor Omidi²  AliAshraf Mehrabi³  Alireza Etminan⁴  Alireza Pour-Aboughadareh⁵ 

[1] Botany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, Finland;Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj P.O. Box 31587-77871, Iran;Department of Agronomy and Plant Breeding, Ilam University, Ilam P.O. Box 69315516, Iran;Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, Iran;Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3183964653, Iran;

关键词: Aegilops spp.;    oxidative stress;    gene expression;    physio-chemical properties;    enzyme activity;   

DOI  :  10.3390/agriculture10090415

来源: DOAJ

【 摘 要 】

Previous studies have revealed that some wild wheat accessions respond well to water deficit treatments and have a good potential in terms of photosynthetic parameters, root system architecture, and several physiological properties. However, the biochemical responses and molecular mechanisms of antioxidant-encoding genes remain to be elucidated. Herein, we investigated the most tolerant accessions from A. crassa, Ae. tauschii, and Ae. cylindrica previously identified from a core collection in previous studies, along with a control variety of bread wheat (T. aestivum cv. Sirvan) through measuring the shoot fresh and dry biomasses; the activities of antioxidant enzymes (including ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX), and peroxidase (POD)); and the relative expression of CAT, superoxide dismutase (MnSOD), and GPX and APX genes under control and water deficit conditions. Water deficit stress caused a significant decrease in the shoot biomasses but resulted in an increase in the activity of all antioxidant enzymes and relative expression of antioxidant enzyme-encoding genes. Principal component analysis showed a strong association between the shoot dry biomass and the activity of CAT, POD, and APX, as well as MnSOD gene expression. Thus, these traits can be used as biomarkers to screen the tolerant plant material in the early growth stage. Taken together, our findings exposed the fact that Ae. tauschii and Ae. crassa respond better to water deficit stress than Ae. cylindrica and a control variety. Furthermore, these accessions can be subjected to further molecular investigation.

【 授权许可】

Unknown